Along the lagging strand's template, primase builds RNA primers in short bursts. DNA ligase then joins the deoxyribonucleotides together, completing the synthesis of the lagging strand. For possible methods involving primers, see Nucleic acid methods. The Sanger chain termination method of sequencing uses a primer to start the chain reaction. The length of primers is usually not more than 30 usually 18—24  nucleotides, and they need to match the beginning and the end of the DNA fragment to be amplified.
Laurie Holland Most shooters and handloaders know that the primer plays a vital role. However, whilst a complete or partial misfire is obvious, inconsistent performance is more common and frequently hard to quantify, or even recognise. Even when the primer is superbly made and its specification well matched to the cartridge and propellant, it is only one part of the cartridge ignition system.
The consistency and condition of the primer-pocket in the case-head, flash-hole and of course, mechanical aspects of the firearm firing pin energy and protrusion affect performance, sometimes severely.
Does PRIMER really make a difference?
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They are known as "battery-cup" type primers. This is a two-part primer design in which the anvil and primer cup are supported in an external cup. Shotshell primers are larger and shaped differently than metallic centerfire primers. This makes them immediately identifiable.
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Makeup Monday: Primer
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